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The loading efficiency was superior to the yield we achieved, but it did not necessarily reflect the yield of capturing of His-tag protein because a 1:1 protein:aptamer molar ratio of capturing was achieved only by using the beads with the lowest surface aptamer density (157 pmol/mg beads) and the protein:aptamer ratio was reduced to 1:5 when using beads with higher aptamer density of 1037 pmol/mg 34
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