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An appropriate concentration of boron fertilizer solution can significantly improve the germination potential, germination rate, shoot length, root length, germination index, and vitality index of buckwheat seeds, but an excessively high concentration of boron inhibits the germination of buckwheat seeds, as was also found in the case of jute seeds in a previous study ( Arabidopsis seedlings ( Vigna radiata L.) seeds treated with 0.01% boron was significantly shortened, and growth indicators, namely average emergence time, emergence index, root length, plant height, and chlorophyll content were significantly improved

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Collectively, our findings position allithiamine as a biologically active vitamer in vivo and provide a robust experimental framework for future studies addressing vitamer-specific kinetics, regulation, and tissue distribution