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Subsequently, phosphofructokinase-1 (PFK-1) catalyzes the conversion of F6P to fructose-1,6-bisphosphate (F1,6BP), a rate-limiting step in glycolysis.42,43 During the energy payoff phase, F1,6BP is cleaved by aldolase into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate (G3P)
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